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Changes in Muscle Fiber Types during the Growth of Ujumqin Sheep |
XUE Bao-ling;WANG Shui-qing;ZHANG Dong-mei;BORJIGIN Gerelt |
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China |
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Abstract Changes in muscle fiber types in M. biceps femoris of Ujumqin sheep at different moths of age were examined
by histochemical staining. The aim of the experiment was to explore the changing regularity of muscle fiber types during
the growth of Ujumqin sheep. The skeletal muscle was fixed in paraformaldehyde fixative solution and histological slides
were prepared and incubated separately in acidic and alkaline incubation solutions, then washed with barbital sodium buffer
and placed in (NH4)2S solution for further incubation. histological slides were dehydrated with gradient ethanol and finally
mounted with neutral balsam prior to microscopic observation. Results showed that the percentage of type Ⅱa muscle fiber
in the M. biceps femoris exhibited no significant difference between 3 and 6 months as well as between 12 and 18 months of
age (P>0.05) and the highest level was observed in the M. biceps femori of 12-year-old sheep. Type Ⅱb muscle fiber showed
the highest percentage in the M. biceps femori of 18-year-old sheep, which, on the contrary, showed the lowest percentage of
type Ⅰ muscle fiber.
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