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| Optimization of Chondroitin Sulfate Extraction from Bovine Pipes by Ultrasonic-assisted Dual-enzyme Hydrolysis |
| TIAN Jia-chun;YU Qun-li |
| College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract Ultrasonic pretreatment followed by sequential hydrolysis with papain and pepsin was used to extract chondroitin
sulfate from bovine pipes. The optimal pepsin hydrolysis conditions were identified by one-factor-at-a-time and orthogonal
array design methods to be pH 5.9, pepsin dosage 1.4:1000 (m/V), temperature 40 ℃ and hydrolysis time 100 min. Under these
conditions, the extraction yield of chondroitin sulfate was 25.43% with a purity of 87.96%. All quality indicators investigated
completely reached the requirements of relevant national standards for chondroitin sulfate.
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| [1] |
ZHOU Ting, WU Yujie, LU Fangyun, HUANG Jin, WU Haihong, ZHANG Xinxiao, XU Weimin, ZOU Ye, WANG Daoying. Optimization of Ultrasound-Assisted Enzymatic Extraction of Collagen Peptide from Chicken Knee Cartilage and Its Antioxidant Activity[J]. Meat Research, 2021, 35(4): 7-15. |
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2011, Vol. 25 
