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| Fast Determination of Residual Clenbuterol in Pork by Liquid Chromatography with Tandem Mass Spectrometry |
| ZHANG Yan, CHEN Guo, LÜ Yan, WU Yinliang* |
| The Ningbo Academy of Agricultural Sciences, Ningbo 315040, China |
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Abstract A method was developed for determining residual clenbuterol in pork by liquid chromatography with tandem
mass spectrometry. Five grams of pork samples were extracted with ethyl acetate under basic condition. Then, clenbuterol
was extracted from the extract using formic acid solution. After defatting with hexane, the extract was directly used for
determination of clenbuter by liquid chromatography with tandem mass spectrometry (LC-MS-MS) on an Acquity BEH C18
column with a mixture of 0.1% formic acid solution and methanol as the mobile phase under gradient elution conditions. The
mass spectrometer was operated in multiple reaction monitoring (MRM) mode using positive electrospray ionization. The
analyte was quantified with the isotope dilution and internal standard methods. Good linearity was obtained for clenbuterol in
the concentration range of 0.05–10.0 μg/L with correlation coefficient more than 0.999. The recoveries of pork samples were
95.9%–101.5% at fortified levels of 0.25–0.75 μg/kg. The proposed method exhibited a limit of detection of 0.10 μg/kg and
a limit of quantitation of 0.25 μg/kg for clenbuterol. The relative standard deviations of intra-assay and inter-assay precision
were between 3.3% and 4.6%, and between 4.1% and 5.1%, respectively. The method is demonstrated to be suitable for the
fast determination of clenbuterol in pork.
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