The Study of the Immune Effect of Extraction and Purification of IgM from the Mucus of Catfish
1. College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030；2. Department of Food Science,Tianjin Agricultural University, Tianjin, 300384)
Abstract：Abstract: This experiment extract and purify IgM from the mucus of catfish by means of gradient sediment using ammonium sulfate and ion-exchanged Chromatography.The results showed that the best pH for ion-exchanged chromatography was 7.4, the best velocity was 3ml/min, and the concentration of NaCl was 1mol/L.The relative molecular mass of the heavy chaine and light chain of IgM was 78ku and 26ku,respectively,as determined by SDS-PAGE.Trough the experiment of rat Splenic lymphoid cell proliferation, we know that when the concentration of IgM was 6 mg/mL, its immunoregulatory activity was better.
2010, Vol. 24 
