摘要对高效液相色谱法测定火腿肠中盐酸克伦特罗的方法进行优化.对色谱条件进行调整,测定条件为采用ODS-Cia型(150mm X 4.6mm,5μm)色谱柱,流动相为甲醇-0.02mol/L乙酸铵(体积比35:65),流速为1.OmL/min,采用紫外检测器,检测波长为244nm.该方法测定结果的相对标准偏差为1.49%(n=4),平均回收率为94.67%.该法能够满足实验要求.
Abstract：A high performance liquid chromatographic method was proposed to determine clenbuteroli hydrochloride in
sausages. Sample preparation conditions and chromatographic conditions were optimized for rapid and accurate determination
of clenbuteroli hydrochloride. The chromatographic separation was carried out on ODS-C18 column (150 mm×4.6 mm, 5μm)
using a mobile phase made up of 0.02 mol/L ammonium acetate and methanol (65:35, V/V) at a flow rate of 1.0 mL/min. The UV
detector used to detect clenbuteroli hydrochloride was set at 244 nm wavelength. The average spike recovery for clenbuteroli
hydrochloride in a blank sample was 94.67%, and the RSD was 1.49% (n = 4).
2011, Vol. 25 
