Optimized Preparation and Antioxidant Activity of Enzymatic Hydrolysate from Hippocampus abdominals
CHEN Peilu, LI Ming, ZHANG Jiayuan, QIU Fengyan, QIU Xianzhu, LUO Youhua, QI Huanyang, XU Guanghui
1. Xiamen Key Laboratory of Natural Medicines Research and Development, Xiamen Institute of Pharmaceutical Research, Xiamen 361000, China; 2. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350000, China; 3. Xiamen Xiaodeng Aquatic Technology Co. Ltd., Xiamen 361000, China; 4. Xiamen Enci Health Business Co. Ltd., Xiamen 361000, China
Abstract：Objective: To optimize the preparation process of an enzymatic hydrolysate with 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity from Hippocampus abdominalis. Methods: Alcalase, flavor protease, trypsin, papain, trypsin, and pepsin were screened for DPPH scavenging activity, and alcalase was found to be optimal. The optimization of the hydrolysis process was conducted using a combination of single factor experiments and response surface methodology (RSM) based on Box-Behnken design. Hydrolysis time, pH, and enzyme/substrate (E/S) ratio were considered as independent variables. The in vitro antioxidant capacity of the hydrolysate was verified. Results: The optimal hydrolysis conditions were determined as 1:20, 11, 7.581%, 50 ℃ and 3 h for solid/liquid ratio, pH, E/S ratio, temperature and time, respectively. The DPPH scavenging activity of the as-prepared hydrolysate was 64.62%. Conclusion: The optimized hydrolysis process can give peptides with high antioxidant activity from Hippocampus abdominalis.
2023, Vol. 37 
