Comparative Study on Collagen in Muscle and Skin of Giant Salamander
DONG Mengyao, CHEN Dejing, YANG Hui, JIN Wengang
1.College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China; 2.Shaanxi Bio-Resource Key Laboratory, Shaanxi University of Technology, Hanzhong 723001, China
Abstract：In this paper, the basic properties of collagens extracted from the muscle matrix protein and skin of giant salamander were evaluated and compared with each other. Acetic acid treatment followed by pepsin hydrolysis was used to extract collagen, and the molecular mass, spectral characteristics, hydroxyproline content, microstructure and viscosity of the collagens were determined. The results showed that the extraction yields of acid-soluble collagen from the skin and muscle matrix protein were 18.29% and 7.08%, and the extraction yields of collagen from the resulting residues with pepsin were 25.67% and 18.65%, respectively. The content of hydroxyproline was (16.05 ± 0.16)% and (10.49 ± 0.42)% for the acidsoluble collagens from the skin and muscle matrix protein, and (18.04 ± 0.08)% and (8.67 ± 0.12)% for the pepsin-extracted collagens, respectively. The ultraviolet and infrared spectral characteristics of the acid-extracted collagens were basically similar to those of the enzyme-extracted collagens, and more types of acid-extracted collagen were extracted from the skin than from the muscle matrix protein. The enzyme-extracted collagen from the skin showed more clear electrophoretic bands than that from the muscle matrix protein, but the molecular masses of them were similar to each other. Scanning electron microscopic (SEM) observation demonstrated that the reticular structure of acid-extracted collagens from both sources was more complete than that of enzyme-extracted collagens. A significant difference was found in the viscosity of collagens from the two sources (P < 0.05), despite having the same properties as each other. Hence, they can be developed as raw materials for collagen supplement and cosmetics.
董梦尧，陈德经，杨 慧，金文刚. 大鲵肌肉与皮中胶原蛋白比较研究[J]. 肉类研究, 2020, 34(12): 11-17.
DONG Mengyao, CHEN Dejing, YANG Hui, JIN Wengang. Comparative Study on Collagen in Muscle and Skin of Giant Salamander. Meat Research, 2020, 34(12): 11-17.
2020, Vol. 34 
