Abstract:Objective: Chitosan-based coatings with flavonoids extracted from Panax notoginseng leaves were investigated for their effects on quality and shelf life of beef stored at a refrigerated temperature of (4 ± 2) ℃. Methods: The optimal formulation was determined by antioxidant and bacteriostatic tests, and its preservative effect was analyzed by storage experiments. Results: A 1:1 (V/V) mixture of chitosan and flavonoids had excellent antioxidant and antibacterial activities. The pH value, total volatile basic content (TVB-N) content and total plate count of samples coated with the composite preservative remained in the ranges for consumption, and the sensory evaluation was also still in the acceptable range over 10 days of storage. Conclusion: The composite preservative can preserve the quality and prolong the shelf life of beef under refrigerated storage.