Simultaneous Determination of 15 Quinolones and Tetracyclines Antibiotics in Chicken Gizzard and Liver by QuEChERS-High Performance Liquid Chromatography-Mass Spectrometry
WANG Zhiyu, ZHANG Guifang, ZHANG Xiaoyu, ZHANG Yi, ZHANG Tiantian
Yantai Center for Disease Control and Prevention, Yantai 264003, China
Abstract：A method which can simultaneously determine the residues of 11 quinolones and 4 tetracyclines antibiotics in chicken gizzard and liver was established using high performance liquid chromatography-mass spectrometry (HPLC-MS). The samples were pretreated by the widely used QuEChERS (quick, easy, cheap, effective, rugged, safe) method and extracted with glacial acetic acid-acetonitrile-water (1:84:15, V/V), and then extract was cleaned up using Discovery? DSC-18 sorbent, separated using methanol as mobile phase A and 0.1% formic acid in water as mobile phase B by gradient elution, and detected in the multiple reaction monitoring (MRM) mode. There were good linear relationships between the concentrations of the 15 antibiotics in the range of 2–80 ng/mL and their response values (R2 ≥ 0.993 3). The detection limits of this method were 0.5–3.0 μg/kg and the quantitation limits were 1.7–10.0 μg/kg. The average recovery of spiked samples was 75.2%–104.4% with relative standard deviation (RSD) of 2.7% to 8.7%. When 51 chicken gizzard samples and 49 chicken liver samples were randomly chosen and tested by this method, the total detection rate of the 15 antibiotics was 21.38% and their residual levels were 3.48–154.00 μg/kg.
2020, Vol. 34 
