Screening for Important Molecular Markers Affecting Meat Quality of Chahar Sheep Based on Label-Free Proteomics
XIE Yuchun, NAI Rile, YANG Feng, MA Lina, MI Lu, CHE Tianyu, GUO Juntao, SU Xin, ZHAO Cun, WANG Zhixin, LI Jinquan, LIU Zhihong
Engineering Research Center for Goat Genetics and Breeding, Inner Mongolia Autonomous Region, Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Autonomous Region, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China
Abstract：In order to study the difference in the protein compositions of different muscles in Chahar sheep and the functions of differential proteins, the identification and quantitative analysis of proteins in Longissimus dorsi and Gluteus of Chahar sheep were carried out using information dependent acquisition (IDA) and label-free proteomics based on sequential windowed acquisition of all theoretical fragment ions (SWATH). Then, bioinformatics analysis was performed. A total of 1 073 proteins were detected in the two muscles including 140 differential proteins. In total, 116 up-regulated and 24 down-regulated proteins were found in Longissimus dorsi. Gene ontology and pathway enrichment analysis showed that the up-regulated proteins were most abundant in the exosome followed by the cell membrane; they were mainly involved in the molecular functions of intramolecular transferase activity, RNA binding and L-lactate dehydrogenase as well as in the biological processes of carbohydrate metabolism, sarcomere and muscle contraction. The network interaction of these differential proteins showed that muscle fiber component module consists of a variety of proteins, including myosin-11, myosin-1 (MYH1), myomesin-1, myosin-2 (MYH2), myosin-8 (MYH8), myosin light chain kinase-2, myosin light chain 2, troponin T-3, telethonin, and myosin-binding protein C. MYH1, MYH2 and MYH8 are expected to be important molecular markers affecting meat quality.
谢遇春，奈日乐，杨 峰，马丽娜，米 璐，车天宇，郭俊涛，苏 馨，赵 存，王志新，李金泉，刘志红. 基于非标记蛋白质组学技术筛选影响察哈尔羊肌肉品质的重要分子标记[J]. 肉类研究, 2019, 33(11): 1-6.
XIE Yuchun, NAI Rile, YANG Feng, MA Lina, MI Lu, CHE Tianyu, GUO Juntao, SU Xin, ZHAO Cun, WANG Zhixin, LI Jinquan, LIU Zhihong. Screening for Important Molecular Markers Affecting Meat Quality of Chahar Sheep Based on Label-Free Proteomics. Meat Research, 2019, 33(11): 1-6.
2019, Vol. 33 
