Contamination and Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction Typing of Proteus on Fresh Livestock and Poultry Meats in Zhongshan City
CHEN Shanjiao1,2, SUN Lengning1,2, CHEN Shaowan1, WANG Zongyuan1, BI Shuilian1,*
1.School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510006, China; 2.School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, China
Abstract：The purpose of this study was to investigate the Proteus contamination on fresh livestock and poultry meats from a local farmer’s market Zhongshan, Guangdong province and to analyze the genetic relationship of the dominant Proteus mirabilis. A total of 268 samples of fresh livestock and poultry meats were randomly collected. The Proteus contaminating meat samples were detected by polymerase chain reaction (PCR) and biochemical assays, and then the molecular typing and homology of P. mirabilis isolated were analyzed by enterobacterial repetitive intergenic consensus (ERIC)-PCR. The results showed that all types of samples were heavily contaminated with Proteus, ranging from severe to moderate in the decreasing order of chicken, duck and pork. Furthermore, the results of PCR and biochemical identification were consistent. Among the 123 strains of Proteus isolated, 117 were P. mirabilis, with a positive rate of 51.6%. ERIC-PCR genotyping exhibited 3?9 bands for each P. mirabilis isolate, suggesting a good polymorphism. A total of 101 strains of P. mirabilis belonged to the E, I and K clusters, with greater than 70% intra-cluster similarity. ERIC-PCR is suitable for the typing and homology analysis of P. mirabilis, and it is of great significance to evaluate food contamination by Proteus and its traceability.
2018, Vol. 32 
