Abstract：In this study, hyperoxic carbon dioxide solution was used for relieving the hypoxic stress response of tilapia during simulated transportation. Ammonia excretion was restrained by bubbling carbon dioxide or adding tricaine methanesulfonate (MS-222) into oxygenated water, and the effect was concentration dependent. The longest survival time of tilapia was 548 min in carbon dioxide solution (9 mg/L, pH 6.2) and 710 min in MS-222 solution (20 mg/L), respectively. The survival rate of both groups was 100% in oxygenated water after 4 hours of simulated transportation. In contrast, the survival time of the control group, in which fish were transported with oxygenated water without additive, was 362 min, and the survival rate was only 33%. Therefore, carbon dioxide or MS-222 is an effective sedative for live tilapia transportation.
2018, Vol. 32 
