Microfloral Changes of Vacuum Packaged Venison during Chilled Storage
SHI He1, HU Tiejun2,*, QIN Fengxian3, CHEN Wei3, LIU Jing4, WANG Zhaohui5, JIA Dongshu6, WU Jun6, YOU Lixin6, CHEN Haiyan6, YU Yan6, LIU Fang6, MA Jinxi6, YANG Bin6, ZHANG Tiehua7
1. College of Agriculture, Yanbian University, Yanji 133002, China;
2. Jilin Dongao Deer Industry Science and Technology Development Co. Ltd., Changchun 130600, China;
3. Engineering Research Center of Deer Industry of Jilin Province, Changchun 130600, China;
4. Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China;
5. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China;
6. College of Organism and Food, Changchun University of Science and Technology, Changchun 130600, China;
7. College of Quartermaster Technology, Jilin University, Changchun 130012, China
Abstract：The microfloral changes of vacuum packaged venison during chilled storage were examined using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Samples were collected at 5-day intervals during the storage for bacterial DNA extraction using a DNA extraction kit before PCR-DGGE analysis. The band of 16S rDNA V3 region from the DGGE analysis was excised for sequence analysis. The dominant spoilage bacteria at the end of the storage included Cronobacter turicensis, Providencia stuartii, Myroides phaeus, Clostridium lactatifermentans.
