Effects of Nitrite on Cell Yield and Ultrastructure of Clostridium Sporogenes
Dong Qing-li;Tu Kang;Liu Bao-lin;Li Bao-guo
1.Institute of Food and Biological Technology, University of Shanghai for Science and Technology Shanghai 200093; 2.College of Food Science and Technology, Nanjing Agricultural University Nanjing 210095
Abstract：In order to study the bacteriostasis mechanisms of nitrite, different nitrite concentrations (0, 100, 200, 300, 400 and 500 mg·L-1) were added into culture medium of C.sporogenes at lag phase of growing. It indicated that the cell yields of C.sporogenes decreased significantly with nitrite concentrations increased. Moreover, the cell ultrastructures were destroyed seriously from the observations of transmission electron microscope after nitrite addition for about 20h. As a result, cell breakage would be one of reasons of nitrite inhibition on bacteria.
2008, Vol. 22 
