Anticancer Effect in Vitro and Mechanism of n-3 Polyunsaturated Fatty Acids in Intramuscular Phospholipids of Hyla Rabbit
XUE Shan
Engineering Research Center of Fujian Province for Fungal Industry, College of Biological Science and Technology, Minnan Normal University, Zhangzhou 363000, China
Abstract：As an extension of our previous work, the composition of n-3 polyunsaturated fatty acids (PUFAs) in intramuscular phospholipids of Hyla rabbit was simulated in vitro to evaluate their functional characteristics. The sensitivity of various tumor cells to the formulation was tested by the methyl thiazolyl tetrazolium method. It turned out that the formulation at doses in the range of 30–180 μmol/L repressed the growth of HepG2, MV3 and A375 tumor cells, HepG2 cells being most sensitive to the formulation. The formulation affected the morphology and quantity of HepG cells in a time- and dose-dependent manner and it induced apoptosis in the tumor cells as determined by Annexin V-fluorescin isothiocyanate and propidium iodide double staining kit. Furthermore, the formulation and the individual PUFAs (α-linolenic acid, eicosapentaenoic acid, docosapentaenoic acid, and docosahexenoic acid (DHA)) could alter oxidative stress in HepG cells and their apoptosis-inducing activity was associated with a significant increase in cellular malondialdehyde accumulation and the relative fluorescence intensity of reactive oxygen species and with a decrease in superoxide dismutase activity. In addition, DHA played a major role in the non-enzymatic oxidation reaction.
2018, Vol. 32 
