Abstract：A method for the determination of sulfanitran in chicken was developed by liquid chromatography-tandem mass
spectrometry. Samples were extracted with formic acid-ethyl acetate (2:98, V/V), and the extract was defatted by hexane
extraction and cleaned up by liquid-liquid partitioning. The analyte was separated on a C18 colum using a mobile phase
consisting of methanol and 5 mmol/L ammonium acetate solution with gradient elution. The mass spectrometry was operated
in negative ion mode using multiple reaction monitoring (MRM) for qualitative and quantitative analysis. The calibration
curve of the method was linear in the range of 2.0–100.0 μg/L, with correlation coefficient more than 0.99. The limit of
detection (LOD) and limit of quantitation (LOQ) were 0.5 μg/kg and 2.0 μg/kg, respectively. At spiked levels of 2.0, 5.0,
10.0 μg/kg, the recoveries ranged from 76.0% to 83.4% with relative standard deviations (RSD) less than 8.7%. This method
proved to be convenient and suitable for the determination of sulfanitran residues in chicken.
