Characterization and Free Radical Scavenging Activity of Pepsin Soluble Collagen from Lophius litulon Skin
MA Huawei1, YANG Huicheng2, WU Yigui1, JIN Yuhua1
1. Fangchenggang Fisheries Technology Extension Station, Fangchenggang 538000, China;
2. Zhejiang Marine Development Research Institute, Zhoushan 316000, China
Abstract：The amino acid composition, ultrastructure and in vitro free radical scavenging activity and in vivo antioxidant activity of pepsin soluble collagen from Lophius litulon skin were investigated. The results showed that the pepsin soluble collagen could potentially be a good drug carrier due to its well-distributed network ultrastructure. The collagen exhibited scavenging activities against superoxide anion and hydroxyl free radicals in a dosage-dependent manner, reaching the highest activity at a concentration of 10 mg/L. In vivo experiments indicated that the collagen remarkably inhibited the formation of malondialdehyde (MDA) and hydroxyproline (Hyp) and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in mouse skin homogenate and the strongest inhibitory effect was observed at 100 mg/ (kg·d). These findings imply that the pepsin soluble collagen has free radical scavenging activity both in vitro and in vivo comparable to that of the peptides derived from Lophius litulon skin collagen obtained in our previous study, which is associated with its amino acid composition and ultrastructure.
