Correlation between Myosin Heavy Chain (MyHC) Gene Expression and Lipid and Conjugated Linoleic Acid Contents in Skeletal Muscle during the Growth of Ujumqin Sheep
XUE Baoling1, LI Liping1, YANG Jing1, GERILRTU2
1.College of Vocational and Technical, Inner Mongolia Agricultural University, Baotou 014109, China; 2.College of Food Science and Technology, Inner Mongolia Agricultural University, Huhhot 010010, China
Abstract:Conjugated linoleic acids (CLA) in sheep meat have a close association with meat quality, affecting meat quality parameters like taste, odor and nutritional value. Given the specific expression of the myosin heavy chain (MyHC) genes in the various types of muscle fibers, the MyHC relative expression levels in the skeletal muscle of Ujumqin sheep were determined by real-time PCR, and its lipid content was measured as well as its CLA content by gas chromatography (GC). The results showed that the relative expression levels of MyHC I and MyHC IIa and lipid and CLA levels exhibited consistent trends with increasing age of sheep. The expression levels of MyHC IIb and MyHC IIx reached the maximum at 12 months of age. The highest lipid content was found in lambs from 6 to 9 months of age; the highest CLA level at 6 months, which then decreased significantly (P < 0.05). From this study, it is concluded that meat quality in Ujumqin lambs slaughtered at 6 months of ages was better than older and younger ones.
薛宝玲,栗丽萍,杨 晶,格日勒图. 不同月龄乌珠穆沁羊MyHC基因与脂肪及共轭亚油酸含量的相关性[J]. 肉类研究, 2019, 33(5): 24-28.
XUE Baoling, LI Liping, YANG Jing, GERILRTU. Correlation between Myosin Heavy Chain (MyHC) Gene Expression and Lipid and Conjugated Linoleic Acid Contents in Skeletal Muscle during the Growth of Ujumqin Sheep. Meat Research, 2019, 33(5): 24-28.