Abstract:An SPE-HPLC method to determine tilmicosin and tylosin residues in pork and chicken has been established.
Samples were extracted with acetonitrile and extracted antibiotic residues were cleaned up using a C18 SPE column, separated
by HPLC, detected with a PDA detector and quantified by external standard method. The established calibration curve showed
good linearity in the range of 30 to 1000 μg/L (r>0.999). The recovery rates of tilmicosin and tylosin from samples spiked at
concentrations of 0.05, 0.10 mg/kg and 0.15 mg/kg were 72.7%–90.8%, with coefficients of variation of 2.6%–8.3%. The limits
of detection (LODs) and limits of quantification (LOQs) for both antibiotics were 30 μg/kg and 50 μg/kg, respectively. The
HPLC method was simple, rapid, accurate, precise and suitable to determine tilmicosin and tylosin residues in pork and chicken.
刘勇军;姜艳彬. 固相萃取-高效液相色谱法测定猪肉与鸡肉中替米考星、泰乐菌素残留量[J]. 肉类研究, 2012, 26(12): 12-15.
LIU Yong-jun;JIANG Yan-bin. Determination of Tilmicosin and Tylosin Residues in Pork and Chicken by SPE-HPLC. Meat Research, 2012, 26(12): 12-15.