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| Rapid Determination of Uric Acid and Purines in Meat and Viscera of Cattle and Sheep from Qinghai Province by High Performance Liquid Chromatography |
| WANG Jingying, BO Haibo*, JI Shengjun, YANG Juan |
| College of Chemistry and Chemical Engineering, Qinghai Normal University, Xining 810008, China |
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Abstract Two analytical methods were developed to determine uric acid and four purines (guanine, hypoxanthine, xanthine and adenine) in meat and viscera of cattle and sheep from Qinghai province. One was based on the simultaneous determination of five individual components by high performance liquid chromatography (HPLC) for subsequent calculation of total uric acid, while the other was based on the enzymatic transformation of all four purines to uric acid for rapid determination of the degradation product by HPLC. In both methods, the chromatographic separation was performed using an Agilent ZorBax Eclipse XDB-C18 column with 7 × 10-3 mol/L KH2PO4-H3PO4 (pH 3.83) as mobile phase, and the analytes were detected at 254 nm. Results shows that the first method achieved good separation and good linearity in a wide concentration range with all correlation coefficients being higher than 0.998 4, and the recoveries were between 92.2% and 119.8% with relative standard deviations (RSD) between 0.78% and 10.43%. Similarly, the second method had a good linearity in the range of 1–70 mg/L with correlation coefficients of 0.999 4. The values of total uric acid obtained by the two methods did not significantly differ from each other as indicated by the matched pairs t-test (P = 0.773 > 0.05). The two methods could be used for the detection of purine compounds in bovine and sheep meat and viscera.
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