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| Rapid Detection of Listeria monocytogenes in Meat Product Using Loop-Mediated Isothermal Amplification Method |
| CHEN Xun，FENG Xue-mei，DU Zheng-ping，TAN Hui-mei，TIAN Wen-wu，LIAO Xiu-zhi，CAO Yi-cheng |
| Guangzhou Huafeng Biotechnology Co. Ltd., Guangzhou 510663, China |
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Abstract Objectives： To establish a method for rapid detection of microbial nucleic acids in food, develop a convenient, rapid and sensitive nucleic acid detection kit, and apply the kit for Listeria monocytogenes detection in meat product. Methods： According to the sequence of the characteristic target gene vir R, specific primer pairs were designed. A detection system was constructed using loop-mediated isothermal amplification and optimized. The established method was analyzed for its specificity and limit of detection, and compared with the conventional methods （based on isolation and subsequent culture）. Results： The detection method and kit developed in this study was specific to Listeria monocytogenes detection in meat product and presented negative results for other related species. The limit of detection was 1.81 × 10^3 CFU/mL, and the sensitivity was consistent with that of the conventional methods. Other advantages such as simple and rapid detection and few needs for instruments were also achieved, thereby deserving to be popularized.
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2011, Vol. 25 
