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| Molecular Biological Detection of Adulteration of Mutton and Beef with Duck |
| WANG Jian-chang;WANG Jin-feng;CHEN Rui-chun;TIAN Zhen-xiang |
| Center of Technology, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China |
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Abstract A molecular biological method was established to detect mutton and beef samples suspected of being adulterated
with duck. Universal primers for the detection of components of animal origin were designed. The PCR amplification products
were sequenced before NCBI database searching for sequence alignment. Primers and probes were synthesized according to
relevant detection standards for the real-time PCR detection of bovine-origin, sheep-origin and duck-origin components. Of 7
tested samples, 4 contained duck-origin components, 2 contained bovine-origin components, and 1 contained sheep-origin
components. This real-time PCR assay was sensitive, specific and rapid.
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2012, Vol. 26 
