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| Mechanism of Biogenic Amine Degradation by a Highly Efficient Biogenic Amine-Degrading Strain, Saccharomyces cerevisiae Z5 |
| FAN Rong, CHEN Li, WANG Li, TAN Xu, JIANG Ziyi, LI Ke, WANG Yuanliang |
| College of Food Science and Technology, Hunan Agricultural University, Changsha 410125, China |
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Abstract The mechanism of biogenic amine degradation by the Saccharomyces cerevisiae wild-type strain Hl and the mutant strain Z5 and related metabolic pathways were explored through transcriptomic and metabolomic analyses. It was found that polyamine oxidase encoded by the FMS1 gene played a crucial role in degrading biogenic amines, particularly spermidine and spermine. Under amine stress, the mutant strain Z5 enhanced its degradation efficiency for biogenic amines by regulating polyamine metabolic pathways. Additionally, the glutathione metabolism pathway was found to play an important role in the amine degradation process of strain Z5. Its polyamine oxidase activity under amine stress was significantly higher than that of the wild-type strain Hl (P < 0.05).
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