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| Metagenomic Analysis of Microbial Contamination and Genetic Information in Cooked Chicken Feet Products |
| SONG Sheng, GUO Kunpeng, HAO Qin, WANG Fang, YAN Li, LI Zheng, ZHANG Jihong |
| 1. Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Hunan Provincial Institute of Product and Goods Quality Inspection, Changsha 410000, China; 2. Miluo City Food and Drug Inspection Institute, Yueyang 414400, China |
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Abstract A traditional culture method and metagenomic analysis were used to determine the level of microbial contamination, community composition and related genetic information in 10 samples of cooked chicken feet products. The results showed that the total bacterial count of sample 7 was 1.3 × 105 CFU/g, which did not meet the requirements of the GB 2726-2016 National Food Safety Standard for Cooked Meat Products. The total bacterial counts of the other samples were all less than 10 CFU/g. The results of microbial DNA concentration and total bacterial count were basically consistent, thus being valid to assess the level of microbial contamination in the samples. The main contaminant bacteria in samples 1–6 and 8–10 were Chlamydia abortus, Escherichia coli, Klebsiella pneumoniae, and Chlamydia psittaci, with relative abundances of 60.87%–75.49%, 13.20%–18.41%, 8.29%–16.41%, and 1.67%–3.67%, respectively. The main contaminant bacteria in sample 7 were Lactobacillus brevis and C. abortus, with relative abundances of about 63% and 8%, respectively. A total of 7 viruses were detected in all samples, the main one being Avian leukemia virus. A total of 12 antibiotic resistance genes and 10 BactMet genes were identified, indicating resistance to various antibiotics and compounds such as tetracycline and acridine orange. A total of 30 virulence factor genes were identified, encoding outer membrane receptor, phospholipase C, and other virulence factors. Acinetobacter baumannii was found to carry 7 antibiotic resistance genes, 5 BactMet genes, and 28 virulence factor genes, being a super antibiotic-resistant and pathogenic bacterium.
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| [1] |
LIU Liang, LI Bingbing, LI Jie, LI Shuangshu, SUN Min, YANG Pengfei, XING Yadong, CHEN Dawei. Analysis of Serotypes, Drug Resistance and Virulence Factors of Salmonella in Raw Meat by Genome-Wide Sequencing[J]. Meat Research, 2024, 38(4): 23-29. |
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2024, Vol. 38 
