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| Optimized Preparation and Antioxidant Activity of Enzymatic Hydrolysate from Hippocampus abdominals |
| CHEN Peilu, LI Ming, ZHANG Jiayuan, QIU Fengyan, QIU Xianzhu, LUO Youhua, QI Huanyang, XU Guanghui |
| 1. Xiamen Key Laboratory of Natural Medicines Research and Development, Xiamen Institute of Pharmaceutical Research, Xiamen 361000, China; 2. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350000, China; 3. Xiamen Xiaodeng Aquatic Technology Co. Ltd., Xiamen 361000, China; 4. Xiamen Enci Health Business Co. Ltd., Xiamen 361000, China |
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Abstract Objective: To optimize the preparation process of an enzymatic hydrolysate with 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity from Hippocampus abdominalis. Methods: Alcalase, flavor protease, trypsin, papain, trypsin, and pepsin were screened for DPPH scavenging activity, and alcalase was found to be optimal. The optimization of the hydrolysis process was conducted using a combination of single factor experiments and response surface methodology (RSM) based on Box-Behnken design. Hydrolysis time, pH, and enzyme/substrate (E/S) ratio were considered as independent variables. The in vitro antioxidant capacity of the hydrolysate was verified. Results: The optimal hydrolysis conditions were determined as 1:20, 11, 7.581%, 50 ℃ and 3 h for solid/liquid ratio, pH, E/S ratio, temperature and time, respectively. The DPPH scavenging activity of the as-prepared hydrolysate was 64.62%. Conclusion: The optimized hydrolysis process can give peptides with high antioxidant activity from Hippocampus abdominalis.
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2023, Vol. 37 
