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| Simultaneous Determination of Oxytetracycline and Microcystin-LR Residues in Freshwater Fish Products |
| DANG Ya-li;HE Hua-ting;WU Yue-guo;WU Xue-qian;ZHAO Zheng-rong |
| 1. Zhejiang Academy of Medical Sciences, Hangzhou 310013, China；
2. College of Life Science, China Jiliang University, Hangzhou 310018, China |
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Abstract A rapid HPLC method for simultaneous determination of oxytetracycline and microcystin-LR (MC-LR)
residues in freshwater fish products has been proposed. Five different sample pretreatment methods were compared.
The limit of detection was 0.1 μg/kg for oxytetracycline and 5 ng/L for microcystin-LR. Calibration curves for both
analytes were linear in the range between 0.1 and 5.0 μ g/mL and the correlation coefficients were 0.995 and 0.998,
respectively. The precision, expressed as relative standard deviation (RSD), was 1.82% for oxytetracycline and 1.68%
for microcystin-LR, and the recoveries from spiked crucian carp muscle samples ranged from 88.1% to 102.8% for
oxytetracycline and from 90.6% to 105% for MC-LR. The analytical results from the HPLC method were very similar
to those obtained with an ELISA assay kit.
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2013, Vol. 27 
