|
|
|
| Determination of Inosine and Its Related Compounds in Chicken by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
| CHEN Chao, WANG Zhen, REN Nan, LIU Mengyao, JI Xiaomeng, LI Yingying, GUO Wenping |
| China Meat Research Center, Beijing 100068, China |
|
|
|
|
Abstract This study aimed to set up an analytical method to detect 5’-adenosine monophosphate (AMP), 5’-inosine monophosphate (IMP), inosine (I), and hypoxanthine (Hx) in chicken by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and to assess its figures of merit. The results showed that the experimental conditions of this method were optimized as follows: samples were homogenized using a vortex mixer by addition of 3% perchloric acid-10% methanol in water followed by centrifugation, and the supernatant extract was purified with a PEIME HLB cartridge after being adjusted to pH 5.0. The analytes were separated on an SB-Aq column (3.0 mm × 100 mm, 1.8 μm) using a mobile phase consisting of 0.1% formic acid in water and acetonitrile by gradient elution at a flow rate of 0.3 mL/min, detected in the positive ion multiple reaction monitoring (MRM) mode, and quantified by the external standard method. The calibration curves for the four nucleoside components had good linearity with correlation coefficients of greater than 0.996. The spiked recoveries ranged between 79.63% and 91.08%, with relative standard deviations (RSDs) of 1.0%–4.5% (n = 6).
|
|
|
|
|
|
|
|
|
|
|
2021, Vol. 35 
