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| A Review of the Application of Real-Time Polymerase Chain Reaction for Quantitative Detection of Animal-Derived Ingredients in Meat Products |
| MA Bingcun, CUI Xuewen, LI Yanxin, WANG Can, HE Qiaoling, CAO Qianchao, LIU Yang, LIU Zheng, LI Zengting, CHEN Xueqiang |
| Sichuan Institute for Food and Drug Control, Chengdu 611731, China |
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Abstract Real-time polymerase chain reaction (RT-PCR) has been widely used in species identification and quantification in meat products with high sensitivity and specificity. There are now several RT-PCR-based quantification strategies for animal-derived ingredients in meat products. Each has its own advantages and disadvantages. However, China does not currently have a national standard for this. In order to provide new ideas for developing an optimal quantitative model, this paper reviews and analyzes the RT-PCR-based quantification strategies and the key factors affecting them such as the form of expression of results, target genes, DNA extractability, DNA degradation, amplification efficiency, reference materials and genome size. This review will be useful for the development of an easy-to-operate, low-cost, widely applicable quantification strategy to help formulate a national standard based on RT-PCR assay.
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2021, Vol. 35 
