|
|
|
| Optimization of Enzymatic Preparation of Dipeptidyl Peptidase-Ⅳ Inhibitory Peptides from Donkey Hemoglobin |
| LIU Dan, WU Xiaotong |
| School of Life Sciences, Inner Mongolia University, Hohhot 010000, China |
|
|
|
|
Abstract Food-derived dipeptidyl peptidase (DPP) Ⅳ inhibitory peptide has become an important direction of research on dietary supplements in recent years. In this study, donkey hemoglobin was hydrolyzed with proteases to produce DPP-Ⅳ inhibitory peptide. The best protease was selected by computer simulation of protein hydrolysis. The effects of hydrolysis time, temperature and enzyme dosage on the degree of hydrolysis and DPP-Ⅳ inhibitory activity were studied. The process conditions were optimized by response surface methodology. The results showed that protease K was the best enzyme for the production of DPP-Ⅳ inhibitory peptide. The optimal enzymatic hydrolysis conditions were as follows: substrate concentration 1%, hydrolysis time 3.15 h, temperature 65 ℃, pH 8.00, and enzyme dosage 400 U/g. The inhibition rate of DPP-Ⅳ by 2 mg/mL of the hydrolysate prepared using these conditions was 53.44%.
|
|
|
|
|
|
|
|
|
|
|
2021, Vol. 35 
