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| Application of DNA Barcoding in Anti-Fraud Identification of Aquatic Products |
| QIU De-yi;HU Jia;LIU De-xing;WEI Xiao-ya;LIU Guo-xiong;YUE Qiao-yun |
| Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, China |
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Abstract This paper reports the application of DNA barcoding techniques to identify whether or not the real species of
insepction samples of import and export aquatic products such as fish is in accordance with the label discriptions. Genomic
DNAs from aquatic products were extracted and amplified by PCR using the universal primer mitochondrial cytochrome C
oxidase subunit Ⅰ (CO Ⅰ) gene. The amplified sequence was aligned with the corresponding ones published in the GenBank
database and was submitted to the Barcode of Life Database (BOLD) for sequence analysis. Sixteen samples of aquatic
products including fish and fishballs were detected by the proposed technique. One of them failed to be amplified by CO Ⅰ
PCR whereas the species of the other 15 was successfully identified. Howoever, 31.25% of the identifed samples were not
the same as described on their labels. As a rapid, simple and effective molecular identification technology, DNA barcoding
can be directly applied to identify the species of meat products like fish.
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| [1] |
WANG Yanli, LIANG Xiuqing, CHEN Qianqian, LI Fangfang, LI Jie, CHEN Keyun, TIAN Qiyan, LI Xia, LIU Yanming. Determination of Eleven N-Nitrosamines in Animal Derived Foods by Gas Chromatography-Tandem Mass Spectrometry after Pass-Through Solid Phase Extraction[J]. Meat Research, 2023, 37(3): 33-39. |
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2013, Vol. 27 
