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| Identification of Cod by Polymerase Chain Reaction and Restriction Endonuclease Digestion |
| LOU Yeqing, HU Yifan, ZHENG Fangyuan, YUAN Wang, CHEN Lixin, SONG Wanru, LI Sufang, PAN Jiarong |
| College of Life Sciences, China Jiliang University, Hangzhou 310018, China |
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Abstract Gadus morhua, an economically important marine fish with high nutritive value, is often adulterated with some inferior and cheap fish species such as Theragra chalcogramma or Argyrosomus argentatus. By comparative analysis of the sequence of mitochondrial gene (COⅠ ) encoding cytochrome c oxidase subunit Ⅰ between Gadus morhua, Theragra chalcogramma and Argyrosomus argentatus, specific primers were designed separately for each fish species. The polymerase chain reaction (PCR) amplicons of COⅠ gene from Gadus morhua and Theragra chalcogramma were both 579 bp in length, while the amplified product of COⅠ gene from Argyrosomus argentatus was 399 bp in length. The PCR product of COⅠ gene from Gadus morhua could not be digested by the BstEII restriction endonuclease, while the PCR product of COⅠ gene from Theragra chalcogramma was digested into two fragments (361 and 218 bp). As a result, Gadus morhua, Theragra chalcogramma and Argyrosomus argentatus could be effectively differentiated from one another.
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2019, Vol. 33 
