|
|
|
| Determination of 20 (Fluoro) Quinolones Residues in Livestock and Poultry Meat by TurboFlow Online Cleanup-Liquid Chromatography-Tandem Mass Spectrometry |
| WANG Bingling1, TIAN Jixin2, XIANG Jialin1, AI Lianfeng1,* |
| 1.Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; 2.College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China |
|
|
|
|
Abstract A new method was developed for the determination of (fluoro)quinolones ((F)QNs) residues in livestock and poultry meat using TurboFlow online cleanup (TFC)-liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples were extracted with methanol-acetonitrile (1 : 1, V/V) mixture. Then the extract was purified on a Cyclone-p column. Subsequently, the analytes were separated on a Hypersil Gold C8 column before being analyzed by tandem mass spectrometry. The calibration curves showed good linearity with R2 > 0.99 in the concentration range from 0.2 to 20.0 ng/mL for cinoxacin, enrofloxacin and danofloxacin, and in the range from 0.1 to 10.0 ng/mL for 17 other (F)QNs. The limit of quantization for enrofloxacin, danofloxacin and cinoxacin was 2 μg/kg, while that for 17 other (F)QNs was 1 μg/kg. Average recoveries of the analytes fortified at three levels ranged from 85.4% to 108.2%, with relative standard deviations (RSDs) between 4.04% and 8.57%. The method was characterized by simplicity, rapidity, high recovery and good repeatability and was suitable for quantitative analysis and confirmation of (F)QNs residues in foods of animal origin.
|
|
|
|
|
|
|
|
|
|
|
2018, Vol. 32 
