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| Determination of Sorbitol in Meat Products by High Performance Liquid Chromatography-Evaporation Light Scattering Detector |
| GAN Ninglan |
| Jiangsu Product Quality Testing and Inspection Institute, Nanjing 210007, China |
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Abstract A high performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD) method was developed for the determination of sorbitol in meat products .The samples were ultrasonically extracted using acetonitrile water (7:3, V/V) as mobile phase and separated on ZORBAX NH2 Agilent column, then detected by evaporative light scattering detector (ELSD). Optimized ELSD detector parameters such as drift tube temperature and nitrogen flow rate were set at 90 ℃ and 2.0 L/min, respectively. The results showed that sorbitol displayed good linearity relationship over the concentration range of 0.2 to 10 mg/mL. The method precision (relative standard deviation, RSD) ranged from 1.28% to 2.02%, and the recovery was between 88.3% and 99.0%. This method has the advantages of simple sample pretreatment, low detection limit and good reproducibility. So it is useful for the determination of sorbitol in meat products.
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