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| Extracting Calcium-binding Peptides from Sheep Bone's Enzymatic Hydrolysates |
| Liu Yu-hua;Ma Li-zhen;Kong Bao-hua;Xin Xin;Li Xue-zhi |
| 1.North East Agriculture University,College of Food Science,Harbin 150030,China; 2.Food Science Department,Tianjin Agricultural College,Tianjin 300384,China |
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Abstract Four commercial protease (pepsin,neutrase,alcalase,papain)were evaluated for hydrolysis of sheep bone.The orthogonal tables of L9(34)was used and 36(4×9)groups hydrolysates were obtained. The content of calcium of the hydrolysates was investigated and the results showed that pepsin hydrolysate has the highest content.The optimum enzyme concentration,substrate concentration,pH,hydrolysis temperature, time were 1600U/g,0.2Kg/L,7.0,55℃and 6.0h,respectively.
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2008, Vol. 22 
