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| Study on the Heating Denatureation Extraction of SOD from Sheep Blood with Response Surface Analysis |
| YU Ying;WANG Xiaoli;XU Guihua |
| 1.Food Department of Agriculture College of Ningxia University,Yinchuan,750021,China;2.Ningxia Agricultural Reclamation Management Bureau,Yinchuan,750001,China |
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Abstract Based on single factor tests,the optimum heating denatureation extraction conditions of SOD from sheep blood were obtained through Box-benhnken central combination design and RSM.The results showed that the optimum heating denatureation extraction conditions of SOD are as follows:heating denatureation temperature 55℃,heating denatureation time 15min and 2%.Under such conditions,the activity exceeds 3 000 U/mg protein was reached.
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2009, Vol. 23 
