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| Effect of Flos Lonicerae Japonicae Extract on the Adhesion Ability of Pseudomonas Strains from Chilled Pork |
| WANG Lin, LI Yuxuan, FAN Wei, LI Henan, GAO Xiaoyue, CHEN Shumin |
| China Meat Research Center, Beijing Academy of Food Science, Beijing 100068, China |
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Abstract The adhesion ability of Pseudomonas strains from chilled pork and the inhibitory effect of Flos Lonicerae Japonicae extract on it was analyzed in this study. A total of 46 strains of Pseudomonas strains were isolated from 15 commercial chilled pork samples, including 22 strains of Pseudomonas putida (47.8%), 10 strains of Pseudomonas fluorescens (21.7%), 6 strains of Pseudomonas aeruginosa (13.0%), and 8 strains of Pseudomonas luteola (17.4%), as identified by 16S rDNA sequence analysis and VITEK 2 system. In total 33 (71.7%) of them were found to have adhesion ability, 17.4% of which had strong adhesion ability, mostly Pseudomonas putida. Flos Lonicerae Japonicae extract could reduce the adhesion ability of the four Pseudomonas species, and had stronger effect on P. putida 5-3 and P. aeruginosa 5-1 in a concentration-dependent manner. At concentrations higher than the minimum inhibitory concentration (MIC), it excerted its effect mainly by inhibiting the growth of bacteria and reducing the production of extracellular polysaccharides. At concentrations lower than the MIC, its excerted its effect mainly by dissolving extracellular polysaccharides, consequently increasing the membrane permeability and reducing uniformity.
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2020, Vol. 34 
