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| Analysis of Bacterial Diversity in Different Parts of Crayfish from Sihong, East China’s Jiangsu Province by Traditional Culture Method and Metagenomic Analysis |
| TANG Chun, ZHU Yongzhi, WU Haihong, SUN Zhilan, ZHU Yunlong, LIU Fang |
| 1.College of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, China; 2.Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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Abstract The bacterial diversity in whole crayfishes and tail muscle, as well as on the surface of crayfishes was investigated using the traditional culture-dependent method and metagenomic analysis in this study. On this basis, the composition of special contaminant bacteria was determined. The results showed that the initial microbial load in whole crayfishes was 8.4 (lg (CFU/g)). Significant variation in microbial community composition was found among the tested samples. The metagenomic analysis showed that the family Flavobacteriaceae was dominant in all samples and Comamonadaceae, Moraxellaceae and Aeromonadaceae were also dominant families in whole crayfishes and tail muscle, as well as on the surface of crayfishes, respectively. At the genus level, Chryseobacterium, Acinetobacter, and Aeromonas were the domain bacteria in whole crayfishes and tail muscle, as well as on the surface of crayfishes, respectively. The relative abundance of Flavobacterium was higher for the three samples.
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2019, Vol. 33 
