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| Establishment of High Performance Liquid Chromatography Method for Determination of Quinolones in Pork Using Imidazolium-Based Ionic Liquids as Mobile Phase Additives |
| TIAN Jing, XIN Keqi, ZHU Zidong, NIU Shubin* |
| The Experimental Teaching Center of Traditional Chinese Medicine and Biotechnology, Beijing City University, Beijing 100094, China |
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Abstract A reversed-phase high performance liquid chromatographic (RP-HPLC) method was established for the determination of quinolones in pork by using imidazolium-based ionic liquids as mobile phase additives. The chromatographic separation of quinolones was performed on a C18 column before ultraviolet detection. The effects of the pH value of ionic liquid and mobile phase composition on the analysis were investigated and optimized. The performance of the method was evaluated in terms of linear range, detection limit and reproducibility. Good baseline separation of norfloxacin and ciprofloxacin was achieved using imidazolium aqueous solution (pH 2.40)-acetonitrile (88:12, V/V) as the mobile phase. Under this condition, the calibration curves of norfloxacin and ciprofloxacin showed a good linearity in the concentration range of 0.05?80.00 μg/mL with R2 greater than 0.999 0, and the detection limits were 0.014 and 0.022 μg/mL, respectively. The intra-day and inter-day precision of migration time and peak area were less than 1.50% for both quinolones, which met the analytical requirements. The average recoveries at three spiked levels (2, 5, 10 mg/kg) ranged from 95.3%?102.0%. The presented method proved to be precise and useful for the determination of quinolones in pork.
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2017, Vol. 31 
