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| Optimization of the Preparation Process of Functional Fish Cartilage Polysaccharide and Its Safety Evaluation for Consumption |
| WU Ruiyun, GUI Meng, LIU Ziyu, Tharushi S. Shinali, SHANG Nan |
| 1. Research Institute of Agricultural Products Processing, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Engineering, China Agricultural University, Beijing 100083, China; 3. Beijing Fisheries Research Institute, Beijing 100068, China; 4. Community Health Service Center of Beijing Language and Culture University, Beijing 100083, China |
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Abstract Low-molecular-mass chondroitin sulfate (CS) have attracted wide attention due to their better bioavailability and biological activity than highmolecular-mass CS. To obtain low-molecular-mass sulfate chondropolysaccharides with higher anti-colorectal cancer activity, sturgeon chondroitin sulfate (SCS) from the hybrid sturgeon Acipenser schrenckii × Huso dauricus was enzymatically depolymerized. Response surface methodology was used to optimize the enzymatic process based on inhibitory activity against the proliferation of HT-29 cells. The optimal enzymatic conditions were determined as 0.103 IU/mg, 1.005 mg/mL and 87 min for enzyme dosage, substrate concentration, and enzymatic digestion time, respectively. Furthermore, SCS-F2 with low molecular mass and high anticancer activity was prepared by ultrafiltration. In order to explore its safety for consumption, the acute toxicity of SCS-F2 was evaluated by using an animal model. The experimental results showed that SCS-F2 has no adverse effects on body mass, food intake, blood biochemical indexes or visceral tissues while reducing the number of aberrant colonic crypts, indicating that SCS-F2 has inhibitory and preventive effects on colorectal cancer and meets the safety requirements of food and health products. Therefore, SCS-F2 is safe and non-toxic, and has anti-colorectal cancer activity.
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2023, Vol. 37 
