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| Effects of Different Extraction Methods and Drying on Fatty Acids in Venison |
| JIN Chun’ai, CUI Songhuan, WANG Yufang, ZHANG Yuwei, LUO Jing, ZHANG Yu, SUN Yinshi* |
| Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China |
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Abstract The goal of this experiment was to investigate the effects of different extraction methods and drying on fatty
acid composition and contents of venison tenderloin. Fresh and dried samples of venison tenderloin were separated into
two portions. One portion was extracted by reflux extraction with petroleum ether in a Soxhlet extractor, and the other one
was extracted by homogenization in a mixture of chloroform and methanol (2:1, V/V). The two extracts were analyzed
by gas chromatography-mass spectrometry (GC-MS) after methyl esterification with potassium hydroxide in methanol.
A total of 21 fatty acids were detected by the homogenization method, while 14 fatty acids were detected by the Soxhlet
extraction method. The content of saturated fatty acids in the Soxhlet extraction method was significantly higher than that
of the homogenization method, and the content of unsaturated fatty acids was not significantly different, but the content of
polyunsaturated fatty acids was significantly decreased. The content of total fatty acids in venison did not significantly differ
between fresh and dried venison samples. The Soxhlet extraction method produced notable changes in fatty acid composition
and contents. The drying process had little impact on fatty acid composition and contents.
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