Abstract：The inhibitory effect of different volume fractions (V/V) of carvacrol on Pseudomonas putida was investigated through the determination of the growth curve, swarming motility, swimming motility, nucleic acid leakage, the ability to produce extracellular protease and biofilm of P. putida. The results showed that carvacrol at a volume fraction equal to or greater than 0.015% had a significant inhibitory effect on the growth, motility, extracellular protease production and biofilm formation of P. putida (P < 0.01). When the volume fraction of carvacrol was 0.025%, the biofilm production of P. putida was reduced by 86.35% compared with the blank group (P < 0.01). Carvacrol can exert an inhibitory effect on P. putida by inhibiting its growth, biofilm-producing ability and motility, and destroying the permeability of the cell membrane, and thereby resulting in the leakage of a large amount of cellular contents.
2024, Vol. 38 
