Abstract：In this paper, the preparation of chondroitin sulfate (CS) by two-step enzymatic hydrolysis of swine trachea
cartilage was optimized using orthogonal array design. The starting raw material was hydrolyzed sequentially by trypsin
and a special-purpose enzyme preparation. The samples obtained were measured for purity and nitrogen and chloride
contents. The optimal hydrolysis conditions were determined as 40 ℃, 1.5% of enzyme concentration and 2.5 h. Under these
conditions, the average yield of CS was 10.98% and the purity was 85.22%. The optimized process is simple and easy to
operate and can provide useful evidence for future industrial production.
2013, Vol. 27 
