1. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China；
2. Department of Animal Husbandry and Veterinary, Yunnan Agricultural Vocational Technical College, Kunming 650212, China；
3. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
Abstract：Tengchong snowcock, with some unique genetic characteristics, such as white feather, black meat and bone, good
meat quality and high disease resistance, is an indigenous chicken breed discovered in recent years in Tengchong county of Yunnan
Province. In the present study, 140-day-old Tengchong snowcocks were chosen for comparative analysis of protein expression
in thigh and breast muscles by two-dimensional gel electrophoresis (2-DE) as well as identification of the proteins with different
expression levels in both muscles by peptide mass fingerprinting with matrix-assisted laser desorption ionization-time of flight-time
of flight-mass spectrometry (MALDI-TOF/TOF MS). The 2-DE analysis displayed high-resolution clear protein spots distributed
in the the range of pH 4.0 to 7.0, and 1421±34 and 1329 ± 25 spots were observed for thigh and breast muscles, respectively.
The results of comparative analysis illustrated a significant difference in the expression levels of 110 spots in the 2-DE images
of both muscles, of which 56 were highly expressed in thigh muscle while 54 in breast muscle. The protein spots were identified
as lamin-A, phosphoglucomutase 1, beta-enolase, superoxide dismutase, creatine kinase M-type, glyoxylase 1, isoform 1 of
tropomyosin alpha-1 chain, and some hypothetical proteins and uncharacterized proteins, respectively.
2013, Vol. 27 
