The Authentication of Commercial Fish Products using DNA Barcoding Technique
SHI Ruihan1,2, NAN Huizhu1, DING Hongtian3, WANG Jianchang1,2, FU Qi1, SUN Xiaoxia1,2, WANG Jinfeng1, DING Lili4, CUI Jingfang3, LIU Yang3, GUO Chunhai1,2,*
1.Center of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; 2.Hebei Academy of Inspection and Quarantine, Shijiazhuang 050051, China; 3.Institute of Hebei Animal Health Supervision, Shijiazhuang 050081, China; 4.Institute of Genetic Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China
Abstract：This study was conducted to identify the species origin of 91 samples of frozen fish products and highly processed, pre-packaged fish products collected from supermarkets using DNA barcoding technique based on homologous sequence analysis of the COI and 16S rRNA genes. The results confirmed that both COI and 16S rRNA gene sequencing were useful for species authentication of fish products. The species origin of 66 of 79 (83.54%) frozen fish products and 7 of 12 (58.33%) highly processed, pre-packaged fish products was consistent with the product claims, which indicated that some problems such as mislabeling and ambiguous ingredient designation of fish products existed in the fish market. This research can provide technical support for the supervisory authorities concerned .
石蕊寒，南汇珠，丁红田，王建昌，付 琦，孙晓霞，王金凤，丁丽丽，崔景芳，刘 洋，郭春海. DNA条形码技术鉴别市售鱼肉制品真伪[J]. 肉类研究, 2018, 32(2): 40-45.
SHI Ruihan, NAN Huizhu, DING Hongtian, WANG Jianchang, FU Qi, SUN Xiaoxia, WANG Jinfeng, DING Lili, CUI Jingfang, LIU Yang, GUO Chunhai. The Authentication of Commercial Fish Products using DNA Barcoding Technique. Meat Research, 2018, 32(2): 40-45.
2018, Vol. 32 
