Abstract：This study was aimed to investigate the effects of peptides (POM-CP) and zinc-chelating peptides (POMCP-Zn) from pearl oyster mantle collagen on the proliferation and differentiation of the osteoblastic cell line MC3T3-E1. The results of methyl thiazolyl tetrazolium assay (MTT) showed that POMCP-Zn significantly stimulated osteoblastic proliferation in comparison to the control, while POM-CP had no effect on cell proliferation. In addition, POM-CP at 400 μg/mL and POMCP-Zn at 10 μg/mL increased alkaline phosphatase activity 1.31- and 1.48-folds in osteoblastic cells compared with the control. Moreover, POMCP-Zn promoted the production of collagen type I and osteocalcin during osteoblastic differentiation and the formation of bone nodules osteoblastic mineralization. In conclusion, POMCP-Zn exerted a stronger effect than POM-CP on the proliferation and differentiation of MC3T3-E1 and POMCP-Zn is a potential candidate functional factor for osteoporosis prevention.
2018, Vol. 32 
