Antioxidant and Sodium Nitrite Scavenging Activity of Crude Peptide Extracted from Nuodeng Ham
WU Baosen1,2, GU Dahai1,2, WANG Guiying1,2, CHENG Zhibin2, XU Zhiqiang1,2, PU Yuehong1,2, WANG Shanrong3,*, LIAO Guozhou1,2,*
1.College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;2.Livestock Product Processing Engineering and Technology Research Center of Yunnan Provine, Kunming 650201, China;3.College of Yunnan Rural Leader, Yunnan Agricultural University, Kunming 650201, Chi
Abstract：In this experiment, the scavenging capacities against hydroxyl and 2,2-diphenyl-1-picrylhydr-azyl (DPPH) free radicals, ferrous ion chelating activity, lipid oxidation inhibitory activity, and sodium nitrite scavenging capability under normal and simulated gastric conditions of the crude peptide extracted from Nuodeng ham were investigated. Results showed that the percentage scavenging of hydroxyl and DPPH free radicals by the crude peptide at 5.0 mg/mL was 47.81% and 67.10%, respectively, highly significantly different from that by glutathione (GSH) at the same concentration (P < 0.01). The percentage chelating of ferrous ion by the crude peptide at 5.0 mg/mL was 31.38%, not significantly different from that by GSH at the same concentration (P > 0.05). The percentage inhibition of lipid oxidation by the crude peptide at 25.0 mg/mL at 144 h of reaction was as high as 77.86%, highly significantly different from that by glutathione (GSH) at the same concentration (P < 0.01). Under normal and simulated gastric conditions, the percentage scavenging of sodium nitrite by the crude peptide at 10.0 mg/mL was (84.31 ± 0.77)% and (80.97 ± 2.00)%, respectively, highly significantly different from that by glutathione (GSH) at the same concentration (P < 0.01). Collectively, we concluded that the crude peptide extracted from Nuodeng ham had antioxidant and lipid oxidation inhibitory activities, and also exhibited strong sodium nitrite scavenging capacity.
2017, Vol. 31 
