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Rapid Detection of Nitrate in Aquatic Products by K-model of Characteristic Absorbance Points |
YE Ting;XU Tao;DENG Shanggui |
College of Food and Medicine, Zhejiang Ocean University, Zhoushan 316000, China |
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Abstract Abstract: Nitrate is closely related to the content of nitrosamine, which is known to be toxic and is determined in aquatic products. In this paper, a new sensitive UV spectrophotometry method based on K-model for rapid determination of nitrate in different kinds of samples was developed. The UV characteristic absorbance points of nitrate were selected and optimized. Under the optimized experimental conditions the calibration equation and K-model were obtained. The calibration curve is linear in the concentration range of 1 to 100μg/ ml (R2=0.9973). This method was found to exhibit good accuracy, precision and repeatability. The developed method could be applied to the determination of nitrate in aquatic products and other food products.
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