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| Development of Ready-To-Use Quality Control Samples for Rapid Detection of Salmonella enteritidis in Meat Foods |
| ZHANG Yalun, XU Miaomiao, LIU Yumeng, ZHU Yanbo, YAN Taotao, WANG Zan, ZHANG Jie |
| Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050071, China |
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Abstract In this study, an instant quality control sample for the detection of Salmonella enteritidis in meat products by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was developed. The homogeneity and stability of the quality control sample were verified by culture counting, MALDI-TOF-MS analysis and statistical analysis. The quality control sample was found to be white in color and spherical in shape. In the homogeneity test, the results of culture counting showed F = 0.75, which is less than the critical value, indicating that the homogeneity is consistent. The transport stability test showed that the number of viable bacteria in the quality control sample was stable and grew well at 37 and 25 ℃. The storage stability test showed that the resuscitation rate of the quality control sample was 90.6% after 28 days of storage at ?20 ℃ and 89.0% after 28 days of storage at 4 ℃, indicating that the sample was stable in nature and could be stored stably for a long period of time. Similarly, the results of each MALDI-TOF-MS cycle were stable. In conclusion, the instant quality control sample for the rapid detection of S. enteritidis developed in this study has good homogeneity and stability, and can be used as a positive quality control sample for the detection and quality control of S. enteritidis.
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2023, Vol. 37 
