|
|
Fatty Acid Fingerprinting of Wuzhumuqin, Dorper and Suffolk Sheep |
SU Xin, CHE Tianyu, XIE Yuchun, ZHAO Cun, GUO Juntao, WANG Zhixin, BAO Yongquan, LI Jinquan, LIU Zhihong |
1.The Inner Mongolia Autonomous Region Goat Genetics and Breeding Engineering Technology Research Center, Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. Eastern Wuzhumuqin Animal Disease Prevention and Control Center, Xilingol 026000, China |
|
|
Abstract In this experiment, six Wuzhumuqin, Dorper and Suffolk sheep raised under the same conditions in Inner Mongolia each were selected and slaughtered. Longissimus dorsi muscles were taken for fatty acid fingerprinting by gas chromatography-mass spectrometry (GC-MS). Similarity analysis of the developed fingerprints was carried out using the software of similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (TCM) and principal component analysis (PCA) was performed as well. The results showed that the similarity in the fatty acid fingerprint of Longissimus dorsi was 0.991 for Dorper versus Suffolk sheep, 0.987 for Dorper versus Wuzhumuqin sheep, and 0.994 for Suffolk versus Wuzhumuqin Sheep. A total of 12 peaks were common to the fatty acid fingerprints of Longissimus dorsi of the three breeds. Based on the retention times of the 12 common peaks and the relative peak areas, PCA accurately discriminated these breeds using stearic acid (C18:0) as the reference peak.
|
|
|
|
|
|
|
|
|