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| Determination of Diethylstilbestrol Residues in Chicken and Duck Meat Using QuEChERS Combined with Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| MO Zimei, NING Xin, CHEN Ningzhou, LIN Dan |
| 1.Guangxi-ASEAN Center For Food and Drug Safety Control, Nanning 530021, China; 2.College of Chemistry and Food Science, Yulin Normal University, Yulin 537000, China; 3.Guangxi Food and Drug Evaluation and Inspection Center, Nanning 530021, China |
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Abstract This paper aims to establish a method for the determination of diethylstilbestrol (DES) in chicken and duck meat using QuEChERS sample preparation followed by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The homogenized sample was dispersed in water and then subjected to the extraction of the analyte with acetonitrile. The chromatographic separation were performed on a XBridge Phenyl-C18 column (150 mm × 2.1 mm, 3.5 μm) by gradient elution after dispersive solid-phase extraction purification. The qualitative and quantitative analysis of DES were operated by electrospray ionization mass spectrometry under the negative mode using multiple reaction monitoring (MRM). Acetonitrile and water were used as the mobile phase. The correlation coefficient of the calibration curve was 0. 999 9 in the concentration range of 0.50 to 20.00 ng/mL. The average recoveries at three spiked concentration levels varied from 70.4% to 81.6% with relative standard deviations (RSD) of 3.1% to 3.9%. The limits of detection (LOD, RS/N ≥ 3) and quantitation (LOQ, RS/N ≥ 10) were 0.1 and 0.4 μg/kg, respectively.
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2019, Vol. 33 
